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- Title
Effect of ex vivo culture conditions on immunosuppression by human mesenchymal stem cells.
- Authors
Lee, Myoung Woo; Kim, Dae Seong; Ryu, Somi; Jang, In Keun; Kim, Hye Jin; Yang, Jin Mo; Lee, Doo-Hoon; Lee, Soo Hyun; Son, Meong Hi; Cheuh, Hee Won; Jung, Hye Lim; Yoo, Keon Hee; Sung, Ki Woong; Koo, Hong Hoe
- Abstract
A microarray analysis was performed to investigate whether ex vivo culture conditions affect the characteristics of MSCs. Gene expression profiles were mainly influenced by the level of cell confluence rather than initial seeding density. The analysis showed that 276 genes were upregulated and 230 genes downregulated in MSCs harvested at ~90% versus ~50% confluence (P < 0.05, FC > 2). The genes that were highly expressed in MSCs largely corresponded to chemotaxis, inflammation, and immune responses, indicating direct or indirect involvement in immunomodulatory functions. Specifically, PTGES and ULBP1 were up-regulated in MSCs harvested at high density. Treatment ofMSCs with PTGES or ULBP1 siRNA reversed their inhibition of T-cell proliferation in vitro. The culture conditions such as cell confluence at harvest seem to be important for gene expression profile ofMSCs; therefore, the results of this study may provide useful guidelines for the harvest ofMSCs that can appropriately suppress the immune response.
- Publication
BioMed Research International, 2013, Vol 2013, p154919
- ISSN
2314-6133
- Publication type
Journal Article
- DOI
2013/154919