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- Title
Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass.
- Authors
Wang, Le; Wan, Zi Yi; Bai, Bin; Huang, Shu Qing; Chua, Elaine; Lee, May; Pang, Hong Yan; Wen, Yan Fei; Liu, Peng; Liu, Feng; Sun, Fei; Lin, Grace; Ye, Bao Qing; Yue, Gen Hua
- Abstract
A high-density genetic map is essential for comparative genomic studies and fine mapping of QTL, and can also facilitate genome sequence assembly. Here, a high density genetic map of Asian seabass was constructed with 3321 SNPs generated by sequencing 144 individuals in a F2 family. The length of the map was 1577.67 cM with an average marker interval of 0.52 cM. A high level of genomic synteny among Asian seabass, European seabass, Nile tilapia and stickleback was detected. Using this map, one genome-wide significant and five suggestive QTL for growth traits were detected in six linkage groups (i.e. LG4, LG5, LG11, LG13, LG14 and LG15). These QTL explained 10.5-16.0% of phenotypic variance. A candidate gene, ACOX1 within the significant QTL on LG5 was identified. The gene was differentially expressed between fast- and slow-growing Asian seabass. The high-density SNP-based map provides an important tool for fine mapping QTL in molecular breeding and comparative genome analysis.
- Subjects
GENE mapping; SEA basses; SINGLE nucleotide polymorphisms; FISH genetics; DNA analysis
- Publication
Scientific Reports, 2015, p16358
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep16358