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- Title
Ecto-5'-Nucleotidase (CD73) Regulates Host Inflammatory Responses and Exacerbates Murine Salmonellosis.
- Authors
Alam, M. Samiul; Kuo, Jennifer L.; Ernst, Peter B.; Derr-Castillo, Victoria; Pereira, Marion; Gaines, Dennis; Costales, Matthew; Bigley, Elmer; Williams, Kristina
- Abstract
Food-borne Salmonella spp., are a major cause of hospitalization and death. Adenosine, an important immune regulator of inflammation, limits tissue damage during infection. CD39 (nucleoside triphosphate dephosphorylase) combined with ecto-5'-nucleotidase (CD73) metabolizes ATP to adenosine. We studied the expressions of CD39 and CD73 in tissues, and T helper cells in mice after Salmonella infection and evaluated the role of CD73 in regulating immune responses and bacterial clearance in wild-type and CD73-deficient (CD73 -/-) mice. Both CD39 and CD73 transcript levels declined in the infected wild-type mice. Compared to wild-type mice, tissues from infected CD73-/- mice had significantly higher expression of pro-inflammatory cytokines and reduced anti-inflammatory responses. CD73-/- mice were more resistant to infection and had a greater inflammatory responses and a significantly lower bacterial load in the liver compared to wild-type mice. Thus, CD73 expression attenuates inflammation during murine Salmonellosis and impairs immunity, leading to increased bacterial colonization and prolonged infection.
- Subjects
SALMONELLA diseases; FOODBORNE diseases; NUCLEOTIDASES; INFLAMMATION; LYMPHOCYTE transformation; BACTERIAL diseases; LABORATORY mice
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep04486