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- Title
High-Resolution Melting Analysis for accurate detection of BRAF mutations: a systematic review and meta-analysis.
- Authors
Dong Chen; Yan-Yan Wang; Zheng-Ran Chuai; Jun-Fu Huang; Yun-Xia Wang; Kai Liu; Li-Qun Zhang; Zhao Yang; Da-Chuan Shi; Qian Liu; Qing Huang; Wei-Ling Fu
- Abstract
The high-resolution melting curve analysis (HRMA) might be a good alternative method for rapid detection of BRAF mutations. However, the accuracy of HRMA in detection of BRAF mutations has not been systematically evaluated. We performed a systematic review and meta-analysis involving 1324 samples from 14 separate studies. The overall sensitivity of HRMA was 0.99 (95% confidence interval (CI) = 0.75-0.82), and the overall specificity was very high at 0.99 (95% CI = 0.94-0.98). The values for the pooled positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio were 68.01 (95% CI = 25.33-182.64), 0.06 (95% CI = 0.03-0.11), and1263.76 (95% CI = 393.91-4064.39), respectively. The summary receiver operating characteristic curve for the same data shows an area of 1.00 and a Q* value of 0.97. The high sensitivity and specificity, simplicity, low cost, less labor or time and rapid turnaround make HRMA a good alternative method for rapid detection of BRAF mutations in the clinical practice.
- Subjects
META-analysis; CELLULAR signal transduction; MITOGEN-activated protein kinases; EPIDERMAL growth factor receptors; NUCLEOTIDE sequence; POLYMERASE chain reaction
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep04168