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- Title
Association between Exosomal miRNAs and Coronary Artery Disease by Next-Generation Sequencing.
- Authors
Chang, Sheng-Nan; Chen, Jien-Jiun; Wu, Jo-Hsuan; Chung, Yao-Te; Chen, Jin-Wun; Chiu, Chu-Hsuan; Liu, Chia-Ju; Liu, Meng-Tsun; Chang, Yi-Cheng; Li, Chin; Lin, Jou-Wei; Hwang, Juey-Jen; Lien, Wen-Pin
- Abstract
Background: Among various bio-informative molecules transferred by exosomes between cells, micro RNAs (miRNAs), which remain remarkably stable even after freeze-and-thaw cycles, are excellent candidates for potential biomarkers for coronary artery disease (CAD). Methods: Blood samples were collected from the coronary arteries of 214 patients diagnosed with three-vessel CAD and 140 without CAD. After precipitation extraction, the amounts of exosomes were found to decrease with increased age and three-vessel CAD. Next-generation sequencing was performed to further explore the possible relationship between exosomal miRNAs and CAD. Results: Eight exosomal miRNAs showed altered expression associated with CAD. The up-regulated miRNAs in CAD were miRNA-382-3p, miRNA-432-5p, miRNA-200a-3p, and miRNA-3613-3p. The down-regulated miRNAs were miRNA-125a-5p, miRNA-185-5p, miRNA-151a-3p, and miRNA-328-3p. Conclusion: We successfully demonstrated particular exosomal miRNAs that may serve as future biomarkers for CAD.
- Subjects
CORONARY artery disease; NUCLEOTIDE sequencing; MICRORNA; EXOSOMES; CORONARY arteries
- Publication
Cells (2073-4409), 2022, Vol 11, Issue 1, p98
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells11010098