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- Title
Amplification of proopiomelanocortin mRNA in canine skin: preliminary results.
- Authors
Kidney, Carolyn M.; Macdonald, John M.; Angarano, Donna W.; Insalaco, Thomas A.; Kempainnen, Robert J.; Sartin, James L.
- Abstract
Skin and pituitary specimens were obtained from a normal chow-chow cross-bred dog. Total messenger RNA (mRNA) was extracted, reversed transcribed and the proopiomelanocortin (POMC) mRNA was amplified by polymerase chain reaction using canine specific primers. The expected 391 bp amplification product was detected in both canine skin and pituitary samples. Sequencing of this product showed 100% homology to the GenBank sequence for canine PMOC cDNA, and confirmed its remarkable homology to sequences of human, pigtailed macaque, mink, pig, mouse, rat and cow POMC. These preliminary results suggest that transcription of POMC mRNA occurs in canine skin. The nature of resident or nonresident cells transcribing the POMC gene in canine skin remains unknown at this time.
- Subjects
PROOPIOMELANOCORTIN; MESSENGER RNA; VETERINARY dermatology; ANIMAL health; VETERINARY medicine; DOG diseases; ANIMAL diseases; BIOCHEMISTRY
- Publication
Veterinary Dermatology, 2004, Vol 15, Issue 6, p389
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/j.1365-3164.2004.00401.x