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- Title
Phenotypic expression of epigenetic determinant [ ISP <sup>+</sup>] in Saccharomyces cerevisiae depends on the combination of sup35 and sup45 mutations.
- Authors
Aksenova, A. Yu.; Volkov, K. V.; Rovinsky, N. S.; Svitin, A. V.; Mironova, L. N.
- Abstract
Translation fidelity in Saccharomyces yeasts is determined by genetic and epigenetic (prion) factors. A study was made of S. cerevisiae strains containing the nonchromosomal determinant [ ISP +], described earlier. Some of its properties suggest that [ ISP +] is a prion. [ ISP +] is expressed phenotypically as an antisuppressor of two sup35 mutations and can be cured with guanidine chloride (GuHCl). It was shown that sup35 mutants containing [ ISP +] carried additional sup45 mutations. These mutations caused amino acid substitutions in different regions of translation termination factor eRF1, encoded by SUP45. Strains bearing the sup35-25 mutation contained the sup45 mutation that caused amino acid substitution at position 400 of eRF1; strains bearing sup35-10 contained the mutation that altered eRF1 at position 75. Thus, the antisuppressor phenotype of the [ ISP +] strains proved to depend on the interaction of sup35 and sup45 mutations, as well as on the GuHCl-curable epigenetic determinant.
- Subjects
SACCHAROMYCES cerevisiae; YEAST; NONSENSE suppression (Genetics); GENETIC mutation; PHENOTYPES; AMINO acids
- Publication
Molecular Biology, 2006, Vol 40, Issue 5, p758
- ISSN
0026-8933
- Publication type
Article
- DOI
10.1134/S0026893306050104