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- Title
Detection of RAPD markers linked to the everbearing gene in Japanese cultivated strawberry.
- Authors
Sugimoto, T.; Tamaki, K.; Matsumoto, J.; Yamamoto, Y.; Shiwaku, K.; Watanabe, K.
- Abstract
To identify markers for the everbearing gene in strawberries, 199 F1 progeny plants were produced from a cross between ‘Ever Berry’ (a Japanese everbearing strawberry) and ‘Toyonoka’ (a Japanese Junebearing strawberry) as the experimental population. The results of flowering tests produced 97 everbears and 102 Junebears. The chi-square test gave a goodness of fit for the expected ratio of 1 : 1 for everbears to Junebears, suggesting the inheritance of the everbearing trait is controlled by a monogenic dominant gene. RAPD analyses on this trait were carried out using ‘Ever Berry’ and ‘Toyonoka’. Seventy-one primers, which produced 89 polymorphic fragments between the two parents, were identified from a total of 175 primers. Five markers relating to the everbearing trait were selected from 26 of the 199 progeny plants. The remaining 173 seedlings were analysed with these five markers and a linkage map was constructed using all of the 199 F1 progeny plants. The length of this linkage group is 39.7 cM. The closest markers found, OPE07-1 and OPB05-1, are respectively mapped at 11.8 and 15.8 cM on each side of the everbearing gene.
- Subjects
JAPAN; STRAWBERRIES; GENES; PLANT genetics; CHI-squared test
- Publication
Plant Breeding, 2005, Vol 124, Issue 5, p498
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2005.01144.x