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- Title
MOLECULAR CHARACTERIZATION OF Staphylococcus aureus ISOLATES RECOVERED FROM NATURAL CASES OF SUBCLINICAL MASTITIS IN CHOLISTANI CATTLE AND THEIR ANTIBACTERIAL SUSCEPTIBILITY.
- Authors
Qayyum, Abdul; Khan, Jawaria Ali; Hussain, Riaz; Khan, Ahrar; Avais, Muhammad; Ahmad, Nisar; Hassan, Mohammad Farooque
- Abstract
Subclinical mastitis primarily with microbial etiology is amenable to different antibiotic therapy and the outcome is doubtful due to the association of a variety of microorganisms, host-specificity and continual evolution of different antibiotic-resistant strains of microorganisms. Therefore, the present study was ascertained to investigate the presence, genotypic characteristics of Staphylococcus aureus isolates recovered from subclinically mastitic Cholistani cattle and the effectiveness of different antibiotics against these pathogens by disc diffusion technique. For this purpose milk samples were collected from a total of 1457 lactating Cholistani cattle and screened for mastitis using California Mastitis Test. All the positive samples were processed for culturing. Staphylococcus aureus isolates isolated and identified on the basis of colony characteristics, coagulase test, biochemical features and amplification of spa (spa-X) and coagulase (coa) genes. The results of PCR revealed that amplification of spa (spa-X) gene yielded different PCR products (400bp and 350bp) while coagulase (coa) produced different products size (390bp, 500bp, and 600bp) indicating genetic variation within and among different herds of the cattle. Moreover, results of this study showed that the spa (spa-X) gene present in coagulase positive (179) and coagulase negative (4) S. aureus isolates. S. aureus isolates were fully sensitive (88%) to amoxicillin, followed by enrofloxacin (78%) and highly resistant to penicillin (65%) and cephradine (100%). It is therefore concluded that S. aureus isolates were genetically different in the study areas and amoxicillin is the drug of choice for treating subclinical mastitis.
- Subjects
TREATMENT of cattle diseases; BOVINE mastitis; ANTIBIOTICS; AMOXICILLIN; STAPHYLOCOCCUS aureus; POLYMERASE chain reaction; FLUOROQUINOLONES; THERAPEUTICS
- Publication
Pakistan Journal of Agricultural Sciences, 2016, Vol 53, Issue 4, p971
- ISSN
0552-9034
- Publication type
Article