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- Title
Studies Using Antibodies against Filaggrin and Filaggrin 2 in Canine Normal and Atopic Skin Biopsies.
- Authors
Marsella, Rosanna; Ahrens, Kim; Wilkes, Rachel
- Abstract
Simple Summary: In people, skin allergies to environmental factors have been linked to abnormalities in the skin itself. One of these abnormalities in people is a lower amount of a family of skin proteins called filaggrins. This decrease makes the skin more permeable to the absorption of environmental allergens such as dust mites. In our study, we wanted to use a dog model of skin allergies to study the impact of standardized and repeated dust mite exposures on the skin itself. More specifically, we wanted to see how these exposures would affect filaggrin proteins and compare the response of normal and allergic dogs. We found that the skin of allergic dogs overreacted to dust mite exposure by getting thicker and increasing the production of filaggrins compared to the normal dogs. Despite this extra production of filaggrin, the allergic skin showed more breakdown products of filaggrin. We conclude that allergic skin attempts to compensate for the allergic insult but it is not as efficient as normal skin. Filaggrin is important for the skin barrier and atopic dermatitis. Another filaggrin-like protein, filaggrin 2, has been described. We evaluated antibodies against both filaggrins in normal and atopic skin biopsies from dogs before and after allergen challenges (D0, D1, D3 and D10). Filaggrins expression was evaluated by immunohistochemistry and Western blot. We used PCR to investigate changes in filaggrin gene expression. Effects of group (p = 0.0134) and time (p = 0.0422) were shown for the intensity of filaggrin staining. Only an effect of group was found for filaggrin 2 (p = 0.0129). Atopic samples had higher intensity of staining than normal dogs [filaggrin on D3 (p = 0.0155) and filaggrin 2 on D3 (p = 0.0038) and D10 (p < 0.0001)]. Atopic samples showed increased epidermal thickness after allergen exposure (D3 vs. D0, p = 0.005), while normal dogs did not. In atopic samples, significant increased gene expression was found for filaggrin overtime but not for filaggrin 2. Western blot showed an increase in filaggrin 2 on D3. A small size band (15 kD) containing a filaggrin sequence was found in Western blots of atopic samples only. We conclude that atopic skin reacts to allergen exposure by proliferating and increasing filaggrin production but that it also has more extensive filaggrin degradation compared to normal skin.
- Subjects
FILAGGRIN; SKIN biopsy; SKIN proteins; SKIN aging; IMMUNOGLOBULINS; ATOPIC dermatitis; GENE expression
- Publication
Animals (2076-2615), 2024, Vol 14, Issue 3, p478
- ISSN
2076-2615
- Publication type
Article
- DOI
10.3390/ani14030478