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- Title
ANTIMICROBIAL ACTIVITY OF THE CELL FREE SUPERNATANTS OF THE LACTIC ACID BACTERIA ISOLATED FROM FRESH COW CHEESE PRODUCED IN TUZLA REGION.
- Authors
Husejnagić, D.; Hodžić, S.; Avdić, A.; Širanović, S.; Vilušić, M.
- Abstract
Lactic acid bacteria (LAB) play essential role in production of fermented food, being a part of natural microbiota of milk. With their metabolic activity they influence the ripening process of milk, allowing the creation of desirable sensory qualities of the product, while inhibiting the growth of undesirable microorganisms. As a by-product of their metabolism, lactic acid bacteria produce a variety of substances with antimicrobial activity such as organic acids (lactic and acetic acid), hydrogen peroxide, ethanol, diacetyl and bacteriocins. Bacteriocins are antimicrobial peptides or proteins with bactericidal activity on certain microorganisms. In this study, cell free supernatant was isolated from lactic acid bacteria of domestically produced fresh cow cheese. This kind of cheese was produced in traditional way, in households of Tuzla Canton, without adding any starter cultures. Isolation and characterization of LAB found that natural microflora of domestically produced cheese was consisted of bacteria of Lactobacillus and Lactococcus species. Concentrated samples of cell free supernatant were isolated out of ten characterized strains, and their antimicrobial activity was tested against pathogenic bacteria Escherichia coli, Staphylococcus aureus, Listeria monocytogenes and Salmonella enteritidis. The highest inhibitory activity on the growth of pathogenic bacteria showed cell free supernatant isolated from Lactobacillus plantarum 1 and Lactobacillus brevis 1. Sensitivity of the supernatant to proteolytic enzyme was tested by using proteinase K. After exposure of the supernatants to proteinase K, the results showed a significant reduction of inhibitory effect. The results proved the protein structure of the cell free supernatant, or the possible presence of bacteriocins and their bacteriocinogenic activity.
- Publication
Technologica Acta, 2016, Vol 9, Issue 2, p49
- ISSN
1840-0426
- Publication type
Article