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- Title
In vitro propagation from rhizomes, molecular evaluation and podophyllotoxin production in Himalayan May Apple (Sinopodophyllum hexandrum Royle T.S. Ying): an endangered medicinal plant.
- Authors
Sharma, Neha; Thakur, Manisha; Sharma, Pooja; Sharma, Yash Pal; Dutt, Bhupender
- Abstract
The present study reports an optimized single step protocol for high frequency in vitro propagation through rhizome buds, molecular analysis and podophyllotoxin production in Sinopodophyllum hexandrum Royle T.S. Ying. Maximum in vitro establishment of buds (96%) was achieved on 0.2 mg/L BA and 0.1 mg/L GA3. Highest multiplication rate (1:4) was achieved on MS medium fortified with 2.0 mg/L BA and 0.2 mg/L NAA after 5th sub-culture. Shoots with swollen rhizomes when transferred for hardening to pots containing sand: soil: FYM (1:1:1) showed 100% survival after 1 month. Molecular analysis using SCoT markers resulted in 148 amplicons of 100–3000 bp and depicted genetic diversity between experimental plants procured from Kullu, Kinnaur and Lahaul-Spiti, whereas showed 99–100% similarity between mother plants and their tissue culture raised progeny. For podophyllotoxin production, callus was induced from in vivo leaves and petioles as well as in vitro roots and leaves of different experimental sites. Highest callus induction from in vivo and in vitro explants was achieved on MS and B5 medium under dark incubation. HPLC analysis revealed maximum podophyllotoxin production from in vitro roots (3.123% w/w) and its calli (0.267% w/w), initiated from experimental plants of district Kinnaur with 3% glucose in production medium. Elicitation of in vitro root calli of Kullu district with methyl jasmonate resulted in enhancing podophyllotoxin production (0.33% w/w). Key message: The present study reports single step in vitro propagation protocol, molecular diversity and fidelity of mother plants and regenerants and podophyllotoxin production in Sinopodophyllum hexandrum Royle T.S. Ying.
- Subjects
PODOPHYLLOTOXIN; PLANT tissue culture; MEDICINAL plants; PILOT plants
- Publication
Plant Cell, Tissue & Organ Culture, 2022, Vol 149, Issue 1/2, p159
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/s11240-022-02288-3