We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparison of molecular changes in lung cancers in HIV-positive and HIV-indeterminate subjects.
- Authors
Wistuba, Ignacio I.; Behrens, Carmen; Milchgrub, Sara; Virmani, Arvind K.; Jagirdar, Jaishree; Thomas, Billue; Ioachim, Harry L.; Litzky, Leslie A.; Brambilla, Elisabeth M.; Minna, John D.; Gazdar, Adi F.; Wistuba, I I; Behrens, C; Milchgrub, S; Virmani, A K; Jagirdar, J; Thomas, B; Ioachim, H L; Litzky, L A; Brambilla, E M
- Abstract
<bold>Context: </bold>Human immunodeficiency virus (HIV) infection has been associated with an increasing incidence of malignancy, and HIV-infected persons have an increased incidence of primary lung carcinoma compared with the general population.<bold>Objective: </bold>To investigate the molecular changes present in HIV-associated lung tumors and compare them with those present in lung carcinomas arising in HIV-indeterminate subjects ("sporadic tumors").<bold>Design: </bold>Convenience sample.<bold>Subjects: </bold>Archival tissues from 11 HIV-positive persons and from 35 persons of indeterminate HIV status.<bold>Setting: </bold>University-based medical centers and affiliated hospitals.<bold>Main Outcome Measures: </bold>Analysis of frequency of loss of heterozygosity (LOH) and microsatellite alteration (MA) using polymerase chain reaction and 16 polymorphic microsatellite markers at 8 chromosomal regions frequently deleted in lung cancer. Presence of HIV and human papillomavirus (HPV) sequences.<bold>Results: </bold>The overall frequency of LOH at all chromosomal regions tested and the frequencies at most of the individual regions were similar in the 2 groups. Frequency of MA present in the HIV-associated tumors (0.18) was 6-fold higher than in sporadic tumors (0.03) (P<.001). At least 1 MA was present in 10 (91%) of 11 HIV-associated tumors vs 17 (48%) of 35 sporadic tumors (P=.02). Molecular changes were independent of tumor stage and gender. HIV and HPV sequences were not detected in the HIV-associated lung carcinomas.<bold>Conclusions: </bold>Microsatellite alterations, which reflect widespread genomic instability, occur at greatly increased frequency in HIV-associated lung carcinomas. Although the mechanism underlying the development of increased MAs is unknown, it may play a crucial role in the development of many HIV-associated tumors.
- Subjects
LUNG tumors; HIV-positive persons
- Publication
JAMA: Journal of the American Medical Association, 1998, Vol 279, Issue 19, p1554
- ISSN
0098-7484
- Publication type
journal article
- DOI
10.1001/jama.279.19.1554