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- Title
P241 - THE MEASUREMENT OF URINARY 8-HIDROXY- 2 DEOXYGUANOSINE (8-OHDG) LEVEL WITH LC-MS/MS METHOD IN PATIENTS WITH POLYCYSTIC OVARY SYNDROME (PCOS).
- Authors
ÖZDEMİR, Şeyda; SERDAR, Muhittin; SEZER, Sevilay; ÇELİK, H. Tuğrul; TORUN GÜNGÖR, Oya; ÖZDEMİR, Özhan; ATALAY, Cemal; YILDIRIMKAYA, M. Metin
- Abstract
Aim: The aim is to determine whether there is an obvious increased oxidative stress in patients with PCOS in long term. 8-OHdG is a determinant of DNA damage. Thus, the initial purpose of this study is to measure the 8-OHdG level in urine samples in order to detect whether there is an oxidative DNA damage in PCOS cases or not. Materials and Methods: 28 patients with PCOS and 27 healthy women were included in this study. First morning urine samples collected and centrifuged at 3500xg for 5 minutes, and then the urinary 8-OHdG level was measured by LC-MS/MS method. The results were expressed as nmol/L. 8-OHdG/creatinin ratio (nmol/mol) was used to compensate the variation of all nucleoside concentration in urine. The relationship between patients and control group was analyzed by Mann-Whitney-U Test. Diagnosis: The statistical analysis show that there is not a significant difference between the patients with PCOS and the control group in terms of the 8-OHdG/creatinin (nmol/mol) ratio (p= 0,533). Conclusion: According to our research, due to an increase of antioxidant and DNA repair capacity, the urinary 8-OHdG/creatinin ratio as a determinant of oxidative stress in patients with PCOS is not different from the healthy women. However, the urinary 8-OHdG/creatinin ratio can be analyzed in a larger group of patients and can be associated with prospective studies about PCOS. Based upon the review of literature, it can be concluded that the urinary 8-OHdG/creatinin ratio can be used in further studies as a carcinogenic factor.
- Subjects
DEOXYGUANOSINE; DEOXYRIBONUCLEOSIDES; HYDROXY acids; CARBOXYLIC acids; POLYCYSTIC ovary syndrome
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2012, Vol 37, Issue S1, p382
- ISSN
0250-4685
- Publication type
Article