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- Title
Sex identification of pigs using polymerase chain reaction amplification of the amelogenin gene.
- Authors
Shoichiro Sembon; Shun-ichi Suzuki; Dai-ichiro Fuchimoto; Masaki Iwamoto; Tatsuo Kawarasaki; Akira Onishi
- Abstract
SummaryThe amelogenin (AMEL) gene exists on both sex chromosomes of various mammalian species and the length and sequence of the noncoding regions differ between the two chromosome-specific alleles. Because both forms can be amplified using a single primer set, the use of AMELin polymerase chain reaction (PCR)-based methods has facilitated sex identification in various mammalian species, including cattle, sheep and humans. In this study, we designed PCR primers to yield different-sized products from the AMELgenes on the X (AMELX) and Y (AMELY) chromosomes of pigs. PCR amplification of genomic DNA samples collected from various breeds of pigs (European breeds: Landrace, Large White, Duroc and Berkshire; Chinese breeds: Meishan and Jinhua and their crossbreeds) yielded the expected products. For all breeds, DNA from male pigs produced two bands (520 and 350 bp; AMELXand AMELY, respectively), whereas samples from female pigs generated only the 520 bp product. We then tested the use of PCR of AMELfor sex identification of in vitro-produced (IVP) porcine embryos sampled at 2 or 5 to 6 days after fertilization; germinal vesicle (GV)-stage oocytes and electroactivated embryos were used as controls. More than 88% of the GV-stage oocytes and electroactivated embryos yielded a single 520 bp single band and about 50% of the IVP embryos tested produced both bands. Our findings show that PCR analysis of the AMELgene is reliable for sex identification of pigs and porcine embryos.
- Subjects
GENDER differences (Psychology); LABORATORY swine; SEX chromosomes; POLYMERASE chain reaction; RECOMBINANT proteins; EMBRYOLOGY
- Publication
Zygote, 2008, Vol 16, Issue 4, p327
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199408004826