We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Site-specific cleavage of mutant ABL mRNA by DNAzyme is facilitated by peptide nucleic acid binding to RNA substrate
- Authors
Kim, Ji Eun; Yoon, Soojin; Mok, Hyejung; Jung, Woong; Kim, Dong-Eun
- Abstract
Abstract: RNA-cleaving DNAzymes were constructed to target the point mutation in the BCR–ABL transcript that causes imatinib resistance in leukemic cells. We examined the effect of 12mer peptide nucleic acids (PNAs) as facilitator oligonucleotides that bind to RNA substrate at the termini of the DNAzyme to improve DNAzyme-mediated cleavage of full-length RNA. When imatinib-resistant cells were transfected with the facilitator PNA and DNAzyme, DNAzyme activity was enhanced and the cells were sensitized to imatinib treatment. Thus, facilitator PNA may be used to enhance activity of antisense oligonucleotide targeting the full-length transcript.
- Subjects
MESSENGER RNA; DEOXYRIBOZYMES; PEPTIDE nucleic acids; POINT mutation (Biology); LEUKEMIA; OLIGONUCLEOTIDES; IMATINIB; CANCER cells
- Publication
FEBS Letters, 2012, Vol 586, Issue 21, p3865
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2012.09.013