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- Title
Avian SERPINB11 gene: a marker for ovarian endometrioid cancer in chickens.
- Authors
Whasun Lim; Ji-Hye Kim; Ahn, Suzie E.; Wooyoung Jeong; Jinyoung Kim; Bazer, Fuller W.; Jae Yong Han; Gwonhwa Song
- Abstract
As serine and cysteine proteinase inhibitors, serpins, such as SERPINB5, cause ovarian, colorectal and pancreatic adenocarcinomas. We identified SERPINB11 as a novel estrogen-induced gene in chickens during oviduct development. The chicken is a unique animal model for research on human ovarian cancer, because it spontaneously develops epithelial cell-derived ovarian cancer as in women. Therefore, this study investigated the expression pattern, CpG methylation status, and miRNA regulation of the SERPINB11 gene in normal and cancerous ovaries from chickens. Our results indicate that SERPINB11 is most abundant in the glandular epithelium of endometrioid adenocarcinoma of cancerous, but not normal, ovaries of hens. In addition, bisulfite sequencing revealed that about 30% of --110 CpG sites are methylated in ovarian cancer cells, whereas --110 CpG sites are demethylated in normal ovarian cells. Next, we determined whether miR-1582 influences SERPINB11 expression via its 3′UTR and found that it does not directly target the 3′UTR of SERPINB11 miRNA. Therefore, it is unlikely that post-transcriptional regulation influences SERPINB11 expression in the chicken ovary. On the other hand, in human ovarian cancer cells such as OCR-3, KO-3 and PA-1 cells, remunerative SERPINB11 protein was predominant in the cytoplasm and had a similar expression pattern to that in chicken ovarian cancer cells. Collectively, these results suggest that SERPINB11 is a bio marker for chicken ovarian endometrioid carcinoma that could be used for diagnosis and monitoring effects of therapies for the disease in women.
- Publication
Experimental Biology & Medicine, 2012, Vol 237, Issue 2, p150
- ISSN
1535-3702
- Publication type
Article
- DOI
10.1258/ebm.2011.011250