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- Title
Improving Efficacy of Bacillus thuringiensis against Insects of Different Feeding Habits by Plasmid Transfer Technique of Chitinase.
- Authors
Abdullah, Reda R.; Sukar, Noha A.; Ghanim, N. M.
- Abstract
This study aimed to increase the efficacy of Bacillus thuringiensis (Bt) (most probably kurstaki) against insects of different feeding habits by chitinase gene transfer from chitinolytic bacteria to a Bt strain through conjugal transfer. Nineteen soil samples were collected from five locations in Qassim region, Saudi Arabia to isolate native strains of B. thuringiensis and chitinolytic bacterium (Bacillus subtilis). Chitinase gene was transmitted from B. subtilis (donor strain) to B. thuringiensis (recipient strain) by conjugal transfer. Five recombinants were selected from all appeared recombinants based on their chitinase activity. The selected recombinants and their parents were evaluated as bioinsecticides against the tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae) and the cotton aphid, Aphis gossypii (Homoptera: Aphididae). In case of T. absoluta, B. thuringiensis was more effective than B. subtilis. The recombinants Tr5 and Tr10 were more effective than other treatments. While, in case of A. gossypii; B. subtilis was more effective than B. thuringiensis. Also, the recombinants Tr5and Tr10 were more effective than their parents and other recombinants.
- Subjects
BACILLUS thuringiensis genetics; BACILLUS thuringiensis; BACILLUS (Bacteria); BACTERIAL genetics; RECOMBINANT proteins
- Publication
Egyptian Journal of Biological Pest Control, 2014, Vol 24, Issue 1, p37
- ISSN
1110-1768
- Publication type
Article