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- Title
si- RNA-Mediated Knockdown of PDLIM5 Suppresses Gastric Cancer Cell Proliferation in Vitro.
- Authors
Li, Yanliang; Gao, Yongsheng; Xu, Yue; Sun, Xianjun; Song, Xilin; Ma, Heng; Yang, Mingshan
- Abstract
Gastric cancer is the second most prominent cause of cancer mortality in the world. This study was designed to identify the possible use of si- RNA-mediated PDLIM5 gene silencing as a therapeutic tool for gastric cancer. Expression levels of PDLIM5 were detected in several gastric cancer cell lines using Western blot and qRT- PCR. We found PDLIM5 is highly expressed in all cultured gastric cancer cell lines. Small interfering RNA (si- RNA) was then employed to knock down PDLIM5 expression in MGC80-3 gastric cancer cells. Knockdown of PDLIM5 significantly inhibited cell proliferation and colony formation. Moreover, the absence of PDLIM5 in MGC80-3 cells led to S phase cell cycle arrest and apoptosis. This study highlights the critical role of PDLIM5 in gastric cancer cell growth and suggests that si- RNA-mediated silencing of PDLIM5 might serve as a potential therapeutic approach for the treatment of gastric cancer.
- Subjects
STOMACH cancer treatment; CANCER-related mortality; SMALL interfering RNA; CANCER cell culture; HUMAN carcinogenesis; CANCER cell proliferation; GENE silencing; GENE expression
- Publication
Chemical Biology & Drug Design, 2015, Vol 85, Issue 4, p447
- ISSN
1747-0277
- Publication type
Article
- DOI
10.1111/cbdd.12428