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- Title
A novel allele of the P- starvation tolerance gene OsPSTOL1 from African rice ( Oryza glaberrima Steud) and its distribution in the genus Oryza.
- Authors
Pariasca-Tanaka, Juan; Chin, Joong; Dramé, Khady; Dalid, Cheryl; Heuer, Sigrid; Wissuwa, Matthias
- Abstract
Key message: We have developed allele-specific markers for molecular breeding to transfer the PSTOL1 gene from Kasalath to African mega-varieties, including NERICAs, to improve their tolerance to P-deficient soil. Abstract: The deficiency of phosphorus (P) in soil is a major problem in Sub-Saharan Africa due to general nutrient depletion and the presence of P-fixing soils. Developing rice cultivars with enhanced P efficiency would, therefore, represent a sustainable strategy to improve the livelihood of resource-poor farmers. Recently the Pup1 locus, a major QTL for tolerance to P deficiency in soil, was successfully narrowed-down to a major gene, the protein kinase OsPSTOL1 ( P- starvation tolerance), which was found to be generally absent from modern irrigated rice varieties. Our target is to improve the tolerance of African mega-varieties to P deficiency through marker-assisted introgression of PSTOL1. As a first step, we have determined the Pup1 haplotype and surveyed the presence or absence of PSTOL1 and other genes of the Pup1 locus in African mega-varieties, NERICAs (New Rice for Africa) and their Oryza glaberrima parents. Here, we report the presence of a novel PSTOL1 allele in upland NERICAs that was inherited from the O. glaberrima parent CG14. This allele showed a 35 base-pair substitution when aligned to the Kasalath allele, but maintained a fully conserved kinase domain, and is present in most O. glaberrima accessions evaluated. In-silico and marker analysis indicated that many other genes of the Kasalath Pup1 locus were missing in the O. glaberrima genome, including the dirigent-like gene OsPupK20- 2, which was shown to be downstream of PSTOL1. We have developed several allele-specific markers for the use for molecular breeding to transfer the PSTOL1 gene from Kasalath to African mega-varieties, including NERICAs.
- Subjects
ALLELES in plants; RICE genetics; PLANTS; BIOMARKERS; PLANT gene mapping; RICE varieties; RICE field irrigation
- Publication
Theoretical & Applied Genetics, 2014, Vol 127, Issue 6, p1387
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-014-2306-y