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- Title
Identification of flanking SSR markers for a major rice gall midge resistance geneGm1and their validation.
- Authors
Biradar, S.K.; Sundaram, R.M.; Thirumurugan, T.; Bentur, J.S.; Amudhan, S.; Shenoy, V.V.; Mishra, B.; Bennett, J.; Sarma, N.P.
- Abstract
Host-plant resistance is the preferred strategy for management of Asian rice gall midge (Orseolia oryzae), a serious pest in many rice-growing countries. The deployment of molecular markers linked to gall midge resistance genes in breeding programmes can accelerate the development of resistant cultivars. In the present study, we have tagged and mapped a dominant gall midge resistance gene,Gm1, from theOryza sativacv. W1263 on chromosome 9, using SSR markers. A progeny-tested F2 mapping population derived from the cross W1263/TN1 was used for analysis. To map the gene locus, initially a subset of the F2 mapping population consisting of 20 homozygous resistant and susceptible lines each was screened with 63 parental polymorphic SSR markers. The SSR markers RM316, RM444 and RM219, located on chromosome 9, are linked toGm1at genetic distances of 8.0, 4.9 and 5.9 cM, respectively, and flank the gene locus. Further, gene/marker order was also determined. The utility of the co-segregating SSR markers was tested in a backcross population derived from the cross Swarna/W1263//Swarna, and allelic profiles of these markers were analysed in a set of donor rice genotypes possessingGm1and in a few gall midge-susceptible, elite rice varieties.
- Subjects
RICE gall midge; PACHYDIPLOSIS; PLANT-fungus relationships; PLANT ecophysiology; PLANT genetics; GENETICS
- Publication
Theoretical & Applied Genetics, 2004, Vol 109, Issue 7, p1468
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-004-1763-0