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- Title
Sacrificial BSA to block non-specific adsorption on organosilane adlayers in ultra-high frequency acoustic wave sensing.
- Authors
Sheikh, Sonia; Blaszykowski, Christophe; Thompson, Michael
- Abstract
This follow-up study describes the implementation of recently developed cross-linking trichlorosilane surface chemistry with acoustic wave sensing technology for the real-time and label-free detection of biotin/avidin interactions. Biosensing platforms consist of unelectroded piezoelectric quartz resonator discs onto which functionalizable mixed organosilane adlayers are prepared using a new trichlorosilane cross-linker in combination with a shorter monofunctional diluent molecule. Thiolated or aminated biotin probes can next be anchored to the mixed assembly in a single, preactivation-free step through site-specific coupling at pentafluorophenyl ester head moieties. Biosensing properties are assessed at ultra-high frequency (>0.74 GHz) with the highly sensitive electromagnetic piezoelectric acoustic sensor using micromolar buffered solutions of avidin. This biosensor prototype - which generally displays good reproducibility - uses sacrificial bovine serum albumin to block non-specific adsorption. This preliminary work in buffer constitutes an important step towards the development of real-world biosensors able to perform with more demanding biological samples. Copyright © 2013 John Wiley & Sons, Ltd.
- Subjects
BODY surface area; NON-specific adsorption; SOUND waves; TRICHLOROSILANE; CRYSTAL resonators; QUARTZ crystal microbalances; QUARTZ crystals
- Publication
Surface & Interface Analysis: SIA, 2013, Vol 45, Issue 11, p1781
- ISSN
0142-2421
- Publication type
Article
- DOI
10.1002/sia.5322