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- Title
Expression of Peroxisome Proliferator-Activated Receptor-γ<sub>1</sub> and Peroxisome Proliferator-Activated Receptor-γ<sub>2</sub> in Visceral and Subcutaneous Adipose Tissue of Obese Women.
- Authors
Giusti, Vittorio; Verdumo, Chantal; Suter, Michel; Gaillard, Rolf Christian; Burckhardt, Peter; Pralong, Francois
- Abstract
Data regarding the expression of peroxisome proliferator-activated receptor (PPAR)-γ[sub 1] and PPAR-γ[sub 2] in human visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) are conflicting. To clarify this issue, we studied 50 women who had a BMI >35 kg/m² were undergoing gastric reduction surgery. Phenotyping included recording of anthropometric parameters and of a biological profile. Quantification of the expression of PPAR-γ[sub 1] and PPAR-γ[sub 2] in samples of VAT and SAT was performed by real-time RT-PCR. In both SAT and VAT, the level of expression of PPAR-γ[sub 2] were >20-fold that of PPAR-γ[sub 1] (P < 0.001 for both). However, only PPAR-γ[sub 1] was differentially expressed, its levels in SAT being 216 ± 34% those in VAT (P < 0.001). In a stepwise, multivariate regression analysis, the levels of PPAR-γ[sub 1] in both SAT and VAT were the major determinants of waist circumference (R² = 21% for both; P < 0.01). Finally, leptin but not PPARs appeared as the single parameter explaining the largest part of the variability of BMI in our cohort of patients (R² = 22%, P < 0.001). These results are consistent with the putative roles of PPAR-γ[sub 1] and PPAR-γ[sub 2] in carbohydrate metabolism and energy homeostasis, respectively. As such, they constitute an important step toward the identification of potential targets for novel therapeutic strategies in the fields of obesity.
- Subjects
PEROXISOMES; ADIPOSE tissues; OVERWEIGHT persons
- Publication
Diabetes, 2003, Vol 52, Issue 7, p1673
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/diabetes.52.7.1673