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- Title
The Effect of N-Acetyl Cysteine on Biofilm Layer in an Experimental Chronic Otitis Media Model.
- Authors
Çallıoğlu, Elif Ersoy; Berçin, Ali Sami; Başdemir, Gülçin; Kiriş, Muzaffer; Tatar, İlkan; Oğuzhan, Tolga; Tüzüner, Arzu; Müderris, Tuba; Sargon, Mustafa; Korkmaz, Mehmet Hakan
- Abstract
Objective: The aim of this study was to investigate the efficacy of n-acetyl cysteine on the biofilm layer and on the course of the disease in resistant chronic otitis media. Methods: Thirty Wistar albino rats were used. Twenty-five rats were inoculated with 10x6 colonies with Pseudomonas aeruginosa strains through the tympanum. The inoculation was repeated one week later. Then the rats were observed without receiving any treatment. Meanwhile, two rats were lost due to malnutrition. The rats that were induced with chronic otitis media were separated into three groups. In Group 1 (n=18) the right ears of the rats were injected with 0.2% Ciprofloxacin + 0.1% Dexamethasone sodium phosphate + 0.5 mg/mL n-acetyl cysteine solution; in Group 2 (n=18) the left ears of the rats were locally injected with 0.2% Ciprofloxacin+0.1% Dexamethasone sodium phosphate solution. No treatment was applied to the inoculated ears of the rats in Group 3 (n=5, both ears). Group 4 (n= 5, both ears) was the control group with no inoculation or treatment. All rats in all groups were decapitated after a four-week follow-up, and histopathological and scanning electron microscope evaluations were performed. Results: In all rats induced with chronic otitis media, Pseudomonas aeruginosa was identified in the swab cultures taken three weeks after the second inoculation from their external auditory canal. Scanning electron microscope revealed biofilm formation in all chronic otitis media induced groups. No statistically significant differences were seen between Groups 1 and 2 in terms of suppuration levels, fibrosis, inner ear involvement, infection staging and biofilm formation (p>0.05). Suppuration, fibrosis and inner ear involvement were not observed in the control group. Conclusion: The efficacy of n-acetyl cysteine on the biofilm layer in chronic otitis media is controversial due to insufficient number of studies. While no effects of n-acetyl cysteine were found on the biofilm in the histopathological and scanning electron microscopy results of this study, further studies with n-acetyl cysteine at different concentrations are needed on different types of experimental animals to arrive at a conclusion.
- Subjects
ACETYLCYSTEINE; OTITIS media; SCANNING electron microscopes; INNER ear; MIDDLE ear
- Publication
Turkish Archives of Otolaryngology / Türk Otolarengoloji Arsivi, 2019, Vol 57, pS5
- ISSN
0304-4793
- Publication type
Article
- DOI
10.5152/tao.2019.20012