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- Title
Selected metabolites profiling of Orthosiphon stamineus Benth leaves extracts combined with chemometrics analysis and correlation with biological activities.
- Authors
Saidan, Noor Hafizoh; Ridzuan Hamil, Mohd Shahrul; Memon, Abdul Hakeem; Abdelbari, Maha Mansour; Hamdan, Mohammad Razak; Mohd, Khamsah Suryati; Abdul Majid, Amin Malik Shah; Ismail, Zhari
- Abstract
Background: Studies on selected metabolites profiling of Orthosiphon stamineus extracts using chromatographic and spectroscopic techniques combined with chemometric tools have not been fully elucidated. Thus present study was performed to profile selected metabolites in O. stamineus leaves extracts using HPLC and FTIR combined with chemometric tools and correlated with biological activities. Methods: Five different extracts were prepared using three methods; maceration, soxhlet and reflux. The extracts were analyzed using UV-Vis, HPLC and FTIR techniques. Analysis of selected primary and secondary metabolites was also evaluated. The antioxidant and cytotoxic activities of the extracts were evaluated. Chemometric tools were employed to classify the extracts based on HPLC analysis and FTIR fingerprints. Results: The ethanolic extract using maceration characterized high content of phenolics and flavonoids, (rosmarinic acid and eupatorin) with high antioxidant activity. Ethanolic (50 %) and methanolic extracts using soxhlet showed high proteins and glycosaponins. Water extracts using reflux and maceration showed high polysaccharides. Methanolic extract (50 %) using soxhlet and methanolic extract using maceration showed strong cytotoxic effect against MCF7 and HCT116 cell lines, respectively. Antioxidant and cytotoxic activities showed significant correlation with selected primary and secondary metabolites. HPLC fingerprints combined with chemometrics showed the extracts have been clustered based on selected major peaks profile. FTIR fingerprints combined with chemometrics showed that the extracts have been clustered based on protein and polysaccharide contents. Conclusion: Ten different extracts of O. stamineus have showed significant differences in the content of selected primary and secondary metabolites as well as the biological activities. Chemometric tools were able to classify and discriminate the distinctive features of extracts thus can be correlated with the biological activities.
- Subjects
CELLULAR signal transduction; CONFIDENCE intervals; STATISTICAL correlation; ETHANOL; HIGH performance liquid chromatography; INFRARED spectroscopy; IRON compounds; METABOLISM; PHENOLS; POLYSACCHARIDES; PROTEINS; TOXICITY testing; ULTRAVIOLET radiation; PLANT extracts; DATA analysis software; DESCRIPTIVE statistics; ODDS ratio
- Publication
BMC Complementary & Alternative Medicine, 2015, Vol 15, Issue 1, p1
- ISSN
1472-6882
- Publication type
Article
- DOI
10.1186/s12906-015-0884-0