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- Title
Direct visualization of a stratified epithelium reveals that wounds heal by unified sliding of cell sheets.
- Authors
Zhao, Min; Song, Bing; Pu, Jin; Forrester, John V.; McCaig, Colin D.
- Abstract
Observing cells in their original niche is a key link between the information gleaned from planar culture and in vivo physiology and pathology. A new approach combining the transparency of the cornea, Hoffman modulation optics, and digital imaging allowed movements of individual corneal cells to be viewed directly in situ. 3‐Dimensional time‐lapse movies imaging unstained cells within the stratified corneal epithelium during wound healing were made. Tracking cell movements dynamically provided a definitive answer to the long‐standing question: does a stratified epithelium heal by "sliding" of cell sheets as a coherent unit or do individual cells "leap frog" each other at the wound margin? A wound in the corneal epithelium healed primarily by sliding of the whole epithelium, with ~95% of cells moving with similar speed and trajectories and with little change in their relative position. Only 5% of cells changed layers, with equal proportions moving up or down. Epithelial healing in situ occurred in three phases: a latency, migration, and reconstruction phase. This model provides a unique system to study the behaviors of individual cells in their original niche. It shows that cells slide into a wound as a unified unit to heal a stratified epithelium.—Zhao, M., Song, B., Pu, J., Forrester, J. V., McCaig, C. D. Direct visualization of a stratified epithelium reveals that wounds heal by unified sliding of cell sheets. FASEB J. 17, 397–406 (2003)
- Publication
FASEB Journal, 2003, Vol 17, Issue 3, p397
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.02-0610com