We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of Endothelin on Sodium/Hydrogen Exchanger Activity of Human Monocytes and Atherosclerosis-Related Functions.
- Authors
KOLIAKOS, GEORGE; BEFANI, CHRISTINA; PALETAS, KONSTANTINOS; KALOYIANNI, MARTHA
- Abstract
The objective of this article is to investigate the influence of endothelin-1 (ET-1) on human monocyte Na+/H+ exchanger (NHE) activity and on the atherosclerosis-related monocyte functions. ET-1 caused an increase in pHi and in 22Na influx of monocytes. A reversal of ET-1 effect on pHi was observed in the presence of the NHE1 inhibitor, cariporide. In addition, the activation of NHE1 by ET-1 was mediated via protein kinase C (PKC), mitogen-activated protein kinase (MAPK), phosphatidylinositol 3-kinase (PI3K), and NADPH oxidase. Also, a link between ET-1 and nitric oxide (NO) was observed. Furthermore, after ET-1 treatment, an increase of the adhesive capacity, the migration ability on laminin and CD36 expression of monocytes, was observed; using cariporide this increase was abolished. Our results showed that ET-1 induces a signaling pathway with the involvement of PKC, MAPK, PI3K, and NADPH oxidase where NHE1 plays a key role. ET-1 also plays a significant role in atherosclerosis-related functions of human monocytes, via NHE1 activation.
- Subjects
ENDOTHELINS; MONOCYTES; RETICULO-endothelial system; ATHEROSCLEROSIS; MITOGEN-activated protein kinases; PROTEIN kinase C
- Publication
Annals of the New York Academy of Sciences, 2007, Vol 1095, Issue 1, p274
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1397.031