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- Title
α7-Type Nicotinic Acetylcholine Receptor and Prodynorphin mRNA Expression after Administration of (--)-Nicotine and U-50,488H in β-Amyloid Peptide (25-35)--Treated Mice.
- Authors
HIRAMATSU, M; WATANABE, M; BABA, S; KOJIMA, R; NABESHIMA, T
- Abstract
We previously reported that (—)-nicotine and κ-opioid receptor agonists lessened impairment of learning and/or memory in several animal models. Furthermore, these drugs prevented neurodegenerative damage induced by ischemia or β-amyloid peptide (25-35). In the present study, we tested whether (—)-nicotine and U-50,488H prevent delayed-memory impairment induced by β-amyloid peptide (25-35), and changes of expression of α7-type nicotinic acetylcholine receptor mRNA and prodynorphin mRNA. Seven days after treatment with β-amyloid peptide (25-35) (9 nmol/mouse, i.c.v.), memory impairment was observed in the Y-maze test. Memory impairment was prevented when (—)-nicotine (6.16 μmol/kg, s.c.) or U-50,488H (21 μ mol/kg, s.c.) was administered 1 h before, but not 1 h after, β-amyloid peptide (25-35) treatment. There was no change in prodynorphin mRNA or α7-type nicotinic acetylcholine receptor mRNA expression in the hippocampus 10 days after β- amyloid peptide (25-35) treatment alone. Of interest, mRNA expression of not only prodynorphin, but also the α7-type nicotinic acetylcholine receptor, was significantly decreased when U-50,488H was administered 1 h before, but not 1 h after, treatment with β-amyloid peptide (25-35). However, these changes were not observed after the administration of (—)-nicotine. These results suggest that activation of the κ-opioid system, but not α7-type nicotinic receptors has a neuroprotective effect on β-amyloid peptide (25-35)-induced memory impairment, and may be involved in the long-lasting changes in the expression of these mRNAs.
- Subjects
NICOTINIC receptors; MEDICAL research; LABORATORY animals; MESSENGER RNA; GENE expression
- Publication
Annals of the New York Academy of Sciences, 2004, Vol 1025, Issue 1, p508
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1196/annals.1316.063