We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
TH1 and TH2 Cytokines Production and NK Cell Level Assessment in Peripheral Blood of Patients with DDH.
- Authors
Akyol, Sibel; Hancı, Murat
- Abstract
In this study, our aim is; if the studies will quide us in peripheral blood, for the changes in inflammatory cytokine levels we defined before DDH tissue. Twenty-six patients were suggestive of lumbar DDH were included in this study. Control subjects included 14 autopsy cases. From each patient, disc tissues and peripheral blood samples for plasma were collected during the surgery. For the controls, disc samples and blood for plasma by intracardiac puncture were obtained during autopsy. The Flow Cytometry was used to obtain the lymphocyte CD56 (NK). The Luminex was used to obtain IL-2, IL-4, IL-10, IL-12, IFN-gamma, in both plasma and disc tissues. The results were compared between the two groups. Comparing the two groups regarding plasma demonstrated that IL-2, IL-4, IL-12, IFN-gamma were significantly higher than in patients than those of the controls. Likewise, tissue levels of IL-2, IL-4, IL-10, IL-12, TNF-alpha, CD56 were found to be significantly higher in the patients. With respect to the comparison between the plasma disc samples in the patients, plasma showed significant higher levels of IL-2, IL-12 on the other hand IL-4 was found to be significantly higher in the disc samples. Findings suggest that only tissue samples responses in occurring but not blood samples. We don't think our results in peripheral blood will guide us specifically in DDH.
- Subjects
INTERVERTEBRAL disk displacement; CHI-squared test; STATISTICAL correlation; CYTOKINES; IMMUNE system; KILLER cells; U-statistics; DATA analysis software; DIAGNOSIS
- Publication
Indian Journal of Surgery, 2013, Vol 75, Issue 4, p294
- ISSN
0972-2068
- Publication type
Article
- DOI
10.1007/s12262-012-0488-6