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- Title
Coupling S-adenosylmethionine–dependent methylation to growth: Design and uses.
- Authors
Luo, Hao; Hansen, Anne Sofie L.; Yang, Lei; Schneider, Konstantin; Kristensen, Mette; Christensen, Ulla; Christensen, Hanne B.; Du, Bin; Özdemir, Emre; Feist, Adam M.; Keasling, Jay D.; Jensen, Michael K.; Herrgård, Markus J.; Palsson, Bernhard O.
- Abstract
We present a selection design that couples S-adenosylmethionine–dependent methylation to growth. We demonstrate its use in improving the enzyme activities of not only N-type and O-type methyltransferases by 2-fold but also an acetyltransferase of another enzyme category when linked to a methylation pathway in Escherichia coli using adaptive laboratory evolution. We also demonstrate its application for drug discovery using a catechol O-methyltransferase and its inhibitors entacapone and tolcapone. Implementation of this design in Saccharomyces cerevisiae is also demonstrated.
- Subjects
ADENOSYLMETHIONINE; METHYLATION; ENZYMES; ESCHERICHIA coli; METHYLTRANSFERASES
- Publication
PLoS Biology, 2019, Vol 17, Issue 3, p1
- ISSN
1544-9173
- Publication type
Article
- DOI
10.1371/journal.pbio.2007050