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- Title
Metabolites of Siamenoside I and Their Distributions in Rats.
- Authors
Xue-Rong Yang; Feng Xu; Dian-Peng Li; Feng-Lai Lu; Guang-Xue Liu; Lei Wang; Ming-Ying Shang; Yong-Lin Huang; Shao-Qing Cai
- Abstract
Siamenoside I is the sweetest mogroside that has several kinds of bioactivities, and it is also a constituent of Siraitiae Fructus, a fruit and herb in China. Hitherto the metabolism of siamenoside I in human or animals remains unclear. To reveal its metabolic pathways, a high-performance liquid chromatography-electrospray ionization-ion trap-time of flight-multistage mass spectrometry (HPLC-ESI-IT-TOF-MSn) method was used to profile and identify its metabolites in rats. Altogether, 86 new metabolites were identified or tentatively identified, and 23 of them were also new metabolites of mogrosides. In rats, siamenoside I was found to undergo deglycosylation, hydroxylation, dehydrogenation, deoxygenation, isomerization, and glycosylation reactions. Among them, deoxygenation, pentahydroxylation, and didehydrogenation were novel metabolic reactions of mogrosides. The distributions of siamenoside I and its 86 metabolites in rat organs were firstly reported, and they were mainly distributed to intestine, stomach, kidney, and brain. The most widely distributed metabolite was mogroside IIIE. In addition, eight metabolites were bioactive according to literature. These findings would help to understand the metabolism and effective forms of siamenoside I and other mogrosides in vivo.
- Subjects
METABOLITE analysis; LABORATORY rats; HERBS; HIGH performance liquid chromatography; DEGLYCOSYLATION; HYDROXYLATION; DEHYDROGENATION
- Publication
Molecules, 2016, Vol 21, Issue 2, p176
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules21020176