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- Title
The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells.
- Authors
Jun-Jun Fan; Liang-Guo Cao; Tao Wu; De-Xin Wang; Dan Jin; Shan Jiang; Zhi-Yong Zhang; Long Bi; Guo-Xian Pei
- Abstract
Icariin had been reported as a potential agent for osteogenesis, but the doseeffect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 10-9 M to 10-6 M, but at concentrations above 10-5 M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration.
- Subjects
CELL-mediated cytotoxicity; CELL differentiation; CELL proliferation; BONE regeneration; BONE growth; DRUG dosage; MESENCHYMAL stem cells
- Publication
Molecules, 2011, Vol 16, Issue 12, p10123
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules161210123