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- Title
HPLC Separation of All Aldopentoses and Aldohexoses on an Anion-Exchange Stationary Phase Prepared from Polystyrene-Based Copolymer and Diamine: The Effect of NaOH Eluent Concentration.
- Authors
Inoue, Kadumi; Kitahara, Kei-ichi; Aikawa, Yoshihiro; Arai, Sadao; Masuda-Hanada, Takako
- Abstract
To investigate the separations of all aldopentoses (ribose, arabinose, xylose and lyxose) and aldohexoses (glucose, galactose, allose, altrose, mannose, gulose, idose and talose) on the D6 stationary phase prepared by the reaction of chloromethylated styrene-divinylbenzene copolymer and N,N,N',N'-tetramethyl-1,6-diaminohexane, we examined the effect of varying the concentration of the NaOH eluent on the elution orders. Separations of these aldoses were achieved using a 20 mM NaOH eluent. The elution behaviors of the aldoses were probably due to not only the individual pKa values, but also the chemical structures of the cyclic aldoses.
- Subjects
SEPARATION (Technology); HIGH performance liquid chromatography; ANIONS; POLYSTYRENE; COPOLYMERS; AMINES
- Publication
Molecules, 2011, Vol 16, Issue 7, p5905
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules16075905