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- Title
Structural Modifications as Tools in Mechanistic Studies of the Cleavage of RNA Phosphodiester Linkages.
- Authors
Lönnberg, Harri
- Abstract
The cleavage of RNA phosphodiester bonds by RNase A and hammerhead ribozyme at neutral pH fundamentally differs from the spontaneous reactions of these bonds under the same conditions. While the predominant spontaneous reaction is isomerization of the 3',5'‐phosphodiester linkages to their 2',5'‐counterparts, this reaction has never been reported to compete with the enzymatic cleavage reaction, not even as a minor side reaction. Comparative kinetic measurements with structurally modified di‐nucleoside monophosphates and oligomeric phosphodiesters have played an important role in clarification of mechanistic details of the buffer‐independent and buffer‐catalyzed reactions. More recently, heavy atom isotope effects and theoretical calculations have refined the picture. The primary aim of all these studies has been to form a solid basis for mechanistic analyses of the action of more complicated catalytic machineries. In other words, to contribute to conception of a plausible unified picture of RNA cleavage by biocatalysts, such as RNAse A, hammerhead ribozyme and DNAzymes. In addition, structurally modified trinucleoside monophosphates as transition state models for Group I and II introns have clarified some features of the action of large ribozymes.
- Subjects
RIBONUCLEASE A; RNA; CATALYTIC RNA; DEOXYRIBOZYMES; PHOSPHODIESTERS
- Publication
Chemical Record, 2022, Vol 22, Issue 11, p1
- ISSN
1527-8999
- Publication type
Article
- DOI
10.1002/tcr.202200141