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- Title
Partial suppression of gene encoding proline dehydrogenase enhances plant tolerance to various abiotic stresses.
- Authors
Ibragimova, S.; Kolodyazhnaya, Ya.; Gerasimova, S.; Kochetov, A.
- Abstract
The role of gene of proline dehydrogenase (PDH) in the maintenance of stress tolerance was investigated using the model transgenic plants of tobacco ( Nicotiana tabacum L.) carrying an antisense suppressor of PDH gene (a fragment of Arabidopsis PDH gene under the control of cauliflower mosaic virus 35S promoter in antisense orientation) and notable for a low activity of PDH and elevated content of proline. The progeny of transgenic plants belonging to the 5th generation (T) with partially suppressed PDH activity was more resistant to various types of stress as compared with the control plants of tobacco, cv. Petit Havana SR-1 (SR1). The seedlings of transgenic lines cultured in Petri dishes on agar media supplemented with stress agents were resistant to high NaCl concentrations (200-300 mM) and water deficit simulated by an increased agar content in the medium (14 g/l) as compared to the control seedlings of cv. SR1. Juvenile plants of transgenic lines grown in pots filled with a mixture of vermiculite and perlite also manifested the higher resistance to water deficit and low temperatures (2°C and −2°C) than the control plants. Thus, the partial PDH suppression correlated with an increase in nonspecific resistance to different types of abiotic stress: salinity, water deficit, and low temperatures. Such transgenic lines of tobacco are promising genetic models for thorough investigation of molecular mechanisms of stress resistance in plants.
- Subjects
PLANT genetic engineering; DEHYDROGENASES; EFFECT of stress on plants; TRANSGENIC plants; TOBACCO; CAULIFLOWER mosaic virus; ARABIDOPSIS thaliana
- Publication
Russian Journal of Plant Physiology, 2012, Vol 59, Issue 1, p88
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443712010086