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- Title
14-3-3 proteins are involved in growth, hyphal branching, ganoderic acid biosynthesis, and response to abiotic stress in <italic>Ganoderma lucidum</italic>.
- Authors
Zhang, Tian-Jun; Shi, Liang; Chen, Dong-Dong; Liu, Rui; Shi, Deng-Ke; Wu, Chen-Gao; Sun, Ze-Hua; Ren, Ang; Zhao, Ming-Wen
- Abstract
<italic>Ganoderma lucidum</italic>, which contains many pharmacologically active compounds, is regarded as a traditional medicinal fungus. Nevertheless, the scarcity of basic research limits the commercial value and utilization of <italic>G. lucidum</italic>. As a class of highly conserved, phosphopeptide-binding proteins present in all eukaryotes, 14-3-3 proteins play vital roles in controlling multiple physiological processes, including signal transduction, primary metabolism, and stress responses. However, knowledge of the roles of 14-3-3 proteins in <italic>Basidiomycetes</italic> is sparse. In this article, two homologs of 14-3-3 proteins, encoded by the two distinct genes <italic>GlBmh1</italic> and <italic>GlBmh2</italic>, were distinguished in <italic>G. lucidum</italic>. We found that <italic>GlBmh1</italic> and <italic>GlBmh2</italic> were expressed at various developmental stages, including in vegetative mycelium cultivated on solid medium and in primordia and fruiting bodies. Moreover, we constructed <italic>GlBmh1</italic> single-silenced strains, <italic>GlBmh2</italic> single-silenced strains, and 14-3-3 double-silenced mutants for further study. When <italic>GlBmh1</italic> and <italic>GlBmh2</italic> were inhibited by RNA interference, the growth rate of mycelia was decreased, and the distance between the aerial hyphal branches was reduced; responses to various abiotic stresses such as oxidants and cell wall and osmotic stressors were also changed. Furthermore, the contents of secondary metabolite ganoderic acids (GAs) were increased after <italic>GlBmh1</italic> and <italic>GlBmh2</italic> were simultaneously silenced. Taken together, we provide evidence that implicates potential roles for the two 14-3-3 proteins in affecting growth and GA biosynthesis, thereby providing new insights into the basic functions of 14-3-3 proteins in <italic>G. lucidum</italic>.
- Subjects
14-3-3 proteins; GANODERMA lucidum; ABIOTIC stress; BIOSYNTHESIS; RNA interference
- Publication
Applied Microbiology & Biotechnology, 2018, Vol 102, Issue 4, p1769
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-017-8711-9