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- Title
Isolation and characterization of a cellulolyticGeobacillus thermoleovoransT4 strain from sugar refinery wastewater.
- Authors
Tai, Shang-Kai; Lin, Hsiu-Ping; Kuo, Jimmy; Liu, Jong-Kang
- Abstract
A novel, cellulolytic, bacterial thermophilic strain, T4, was isolated from sugar refinery wastewater in southern Taiwan. This isolate, a Gram-negative, motile, aerobically growing sporulating rod, can secrete thermostable endocellulase (endo-1,4-ß-D-glucanase, EC 3.2.1.4) and hydrolyze carboxymethylcellulose (CMC), phosphoric acid-swollen cellulose, Avicel, filter paper, and salicin. When strain T4 was grown in CMC medium, the cellulolytic enzyme activity in culture supernatants was stable up to 70°C. More than 10% of the original activity was still detectable after heating to 100°C with a pH 7.0 for 1 h. Based on 16S rDNA sequence analysis, DNA base composition, phenotypic and physiological characteristics, as well as DNA-DNA hybridization, strain T4 was classified asGeobacillus thermoleovoransT4 (DSM 14791 = CCRC 17200). We also demonstrated that the type speciesG. stearothermophilus(DSM 22 = ATCC 12980) could hydrolyze amorphous and crystalline (filter paper) celluloses at a rate of 13 and 14%, respectively, in comparison with strain T4.
- Subjects
THERMOPHILIC bacteria; CELLULASE; THERMOPHILIC microorganisms; SEWAGE; BACTERIOLOGY; MICROBIOLOGY
- Publication
Extremophiles, 2004, Vol 8, Issue 5, p345
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-004-0395-2