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- Title
IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors.
- Authors
Sacco, Alessandra; Doyonnas, Regis; LaBarge, Mark A.; Hammer, Mark M.; Kraft, Peggy; Blau, Helen M.
- Abstract
Muscle damage has been shown to enhance the contribution of bone marrow-derived cells (BMDCs) to regenerating skeletal muscle. One responsible cell type involved in this process is a hematopoietic stem cell derivative, the myelomonocytic precursor (MMC). However, the molecular components responsible for this injury-related response remain largely unknown. In this paper, we show that delivery of insulin-like growth factor I (IGF-I) to adult skeletal muscle by three different methods--plasmid electroporation, injection of genetically engineered myoblasts, and recombinant protein injection--increases the integration of BMDCs up to fourfold. To investigate the underlying mechanism, we developed an in vitro fusion assay in which co-cultures of MMCs and myotubes were exposed to IGF-I. The number of fusion events was substantially augmented by IGF-I, independent of its effect on cell survival. These results provide novel evidence that a single factor, IGF-I, is sufficient to enhance the fusion of bone marrow derivatives with adult skeletal muscle.
- Subjects
BONE marrow cells; MUSCLES; STEM cells; SOMATOMEDIN; RECOMBINANT proteins
- Publication
Journal of Cell Biology, 2005, Vol 171, Issue 3, p483
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200506123