We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Myricetin protects keratinocyte damage induced by UV through IκB/NFκb signaling pathway.
- Authors
Xie, Jing; Zheng, Yanyan
- Abstract
Objective: The aim of this study was to evaluate the potential molecular mechanism of myricetin that protecting cells from photodamage. Methods: Myricetin had broadly chemopreventive effects and anti-inflammatory properties. The effect of myricetin was assessed on HaCaT cells. Cell viability assay was carried out. Reactive oxygen species (ROS) level was measured. The expression of pro-inflammatory factor COX2 was determined by real-time PCR and Western blot. The protein levels of p-IκBa and IκBa were determined by Western blot. Results: Myricetin attenuated UV-induced keratinocyte death in a dose-dependent manner as determined by cell viability assay. Pretreatment with myricetin also reduced the UV-induced ROS levels. Myricetin suppresses the upregulation of COX2 induced by UV in keratinocyte as demonstrated by real-time PCR and Western blot. Furthermore, signal transduction studies confirmed that myricetin attenuates the upregulation of COX2 induced by UV via suppression of IκB/NFκB pathways. Conclusion: These results showed that antioxidant property of myricetin can effectively attenuate UV-caused cell damage and suppress the expression of COX2 through the IκB/NFκB signaling pathways. Myricetin had potential protective effects on UV-induced skin cell damages, which might be used in cosmetic and pharmaceutical industries.
- Subjects
MYRICETIN; KERATINOCYTES; ULTRAVIOLET radiation; SKIN abnormalities treatment; INFLAMMATION; COSMETICS; PHARMACEUTICAL industry
- Publication
Journal of Cosmetic Dermatology, 2017, Vol 16, Issue 4, p444
- ISSN
1473-2130
- Publication type
Article
- DOI
10.1111/jocd.12399