We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Bovine Herpesvirus-4 Based Vaccine Provides Protective Immunity against Streptococcus suis Disease in a Rabbit Model.
- Authors
Dong, Nihua; Nichols, Hester; Sun, Qing; Chen, Xiaojun; Zheng, Jiayang; Guan, Zhixin; Zhang, Hailong; Davison, Andrew; Wezel, Yvonne; Li, Zongjie; Li, Beibei; Liu, Ke; Shao, Donghua; Qiu, Yafeng; Sun, Jianhe; Li, Xiangdong; Upton, Mathew; Ma, Zhiyong; Jarvis, Michael A.; Wei, Jianchao
- Abstract
Streptococcus suis (S. suis) is a bacterial pathogen of pigs that has a major animal health and economic impact on the pig industry. Bovine herpesvirus-4 (BoHV-4) is a new virus-based vaccine vector that has been used for the immunogenic delivery of antigens from a variety of pathogens. In the present study, two recombinant BoHV-4-based vectors were evaluated for their ability to induce immunity and protection against S. suis in a rabbit model. The GMD protein is a fusion protein consisting of multiple dominant B-cell epitopes ((B-cell dominant epitopes of GAPDH, MRP, and DLDH antigens) (BoHV-4/GMD)) and the second suilysin (SLY) (BoHV-4/SLY) from S. suis serotype 2 (SS2). Both GMD and SLY delivered by the BoHV-4 vectors were recognized by sera from SS2-infected rabbits. The vaccination of rabbits with the BoHV-4 vectors induced antibodies against SS2, as well as against additional S. suis serotypes, SS7 and SS9. However, sera from BoHV-4/GMD-vaccinated animals promoted a significant level of phagocytic activity by pulmonary alveolar macrophages (PAMs) against SS2, SS7, and SS9. In contrast, sera from rabbits immunized with BoHV-4/SLY induced PAM phagocytic activity against only SS2. In addition, BoHV-4 vaccines differed in the associated level of protection against lethal SS2 challenge, which ranged from high (71.4%) to low (12.5%) for BoHV-4/GMD and BoHV-4/SLY, respectively. These data suggest BoHV-4/GMD as a promising vaccine candidate against S. suis disease.
- Subjects
STREPTOCOCCUS suis; ALVEOLAR macrophages; IMMUNITY; CHIMERIC proteins; BOS; RABBIT diseases; BOVINE viral diarrhea
- Publication
Vaccines, 2023, Vol 11, Issue 5, p1004
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines11051004