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- Title
Development of a Potent Stabilizer for Long-Term Storage of Foot-and-Mouth Disease Vaccine Antigens.
- Authors
Kim, Ah-Young; Kim, Hyejin; Park, Sun Young; Park, Sang Hyun; Kim, Jae-Seok; Park, Jung-Won; Park, Jong-Hyeon; Ko, Young-Joon; Meurens, François
- Abstract
A local virus isolate, O/SKR/JC/2014 (O JC), has been considered as a candidate vaccine strain in the development of a domestic foot-and-mouth disease (FMD) vaccine in Korea. However, producing and preserving a sufficient quantity of intact vaccine antigens from the O JC strain was difficult owing to its distinctive structural instability compared to other candidate vaccine strains. Based on this feature, the O JC strain was adopted as a model virus for the stabilization study to determine the optimal stabilizer composition, which enables long-term storage of the FMD vaccine antigen in both aqueous and frozen phases. In contrast to O JC vaccine antigens stored in routinely used Tris-buffered or phosphate-buffered saline, those stored in Tris-KCl buffer showed extended shelf-life at both 4 °C and −70 °C. Additionally, the combined application of 10% sucrose and 5% lactalbumin hydrolysate could protect O JC 146S particles from massive structural breakdown in an aqueous state for up to one year. The stabilizer composition was also effective for other FMDV strains, including serotypes A and Asia 1. With this stabilizer composition, FMD vaccine antigens could be flexibly preserved during the general production process, pending status under refrigeration and banking under ultrafreezing.
- Subjects
SOUTH Korea; ASIA; FOOT &; mouth disease; ANTIGENS; VACCINES; VACCINE development; LACTALBUMIN
- Publication
Vaccines, 2021, Vol 9, Issue 3, p252
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines9030252