We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Subretinal delivery of immunoglobulin G with gold nanoparticles in the rabbit eye.
- Authors
Mark Pennesi; Eugene de Juan
- Abstract
Abstract Purpose To examine the feasibility of subretinal delivery of immunoglobulin G (IgG) adsorbed onto gold nanoparticles (GNPs) and its histologic distribution in the rabbit retina after the injection. Methods Goat IgG was adsorbed onto GNPs electrostatically. Goat IgG-adsorbed GNPs or buffer with goat IgG was injected into the subretinal space of rabbit eyes and followed up for 3 months by examination of fundus photographs, immunohistochemistry against goat IgG, and transmission electron microscopy (TEM). Human retinal pigment epithelial cells (ARPE-19 cells) were cultured, and cell proliferation with or without GNPs was assayed. Results At 1 week after the subretinal injection of goat IgG-adsorbed GNPs, retinal degeneration was observed in the outer retina, and goat IgG was immunolabeled in the retinal pigment epithelium (RPE) and the photoreceptor cells. TEM showed GNPs located in the outer segments and in the lysosomes in the RPE at 1 month and no apparent cytotoxicity of the RPE. There were no inhibitory effects of GNPs on proliferation of ARPE-19 cells. Conclusions Goat IgG was successfully delivered into photoreceptor cells and RPE using GNPs, though retinal degeneration in the outer retina occurred in this model. This might be an alternative drug delivery method to photoreceptors and RPE.
- Subjects
IMMUNOGLOBULIN G; RETINA; DRUG delivery systems; COLLOIDAL gold; LABORATORY rabbits; FEASIBILITY studies; HISTOLOGY; EYE care
- Publication
Japanese Journal of Ophthalmology, 2009, Vol 53, Issue 3, p249
- ISSN
0021-5155
- Publication type
Article