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- Title
Proteomic analysis of rat retina in a steroid-induced ocular hypertension model: Potential vulnerability to oxidative stress.
- Authors
Yoshito Yamashiro; Akihiro Iwamatsu; Ken-ichi Kariya; Shoichi Sawaguchi
- Abstract
Abstract Purpose  To investigate global protein expression profiles in the retinas of normal and glucocorticoid-induced ocular hypertensive rats by proteomic analysis. Methods  Ocular hypertension was induced by topical application of dexamethasone (DEX) for 4 weeks. Age-matched untreated rats served as controls. Intraocular pressure (IOP) was monitored by an electronic tonometer. Retinal protein expression profiling was carried out by two-dimensional fluorescence difference gel electrophoresis (2-D DIGE). Proteins were identified by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Results  In DEX-treated rats, average IOP was elevated significantly compared with controls. With DEX treatment, levels of four proteins were altered, as revealed by 2-D DIGE and MALDI-TOF mass spectrometry: apolipoprotein A1 (apoA1), a lipid-binding protein, upregulated 1.9-fold, P P P P Conclusions  Downregulation of CRYAA, SOD1, and TPI1, observed here after a short period of DEX-induced ocular hypertension, may be involved in the onset of neural damage in steroid-induced glaucoma.
- Subjects
HYPERTENSION; PROTEOMICS; STEROIDS; OXIDATIVE stress; LABORATORY rats
- Publication
Japanese Journal of Ophthalmology, 2008, Vol 52, Issue 2, p84
- ISSN
0021-5155
- Publication type
Article