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- Title
AnAnopheles gambiaesalivary gland promoter analysis inDrosophila melanogasterandAnopheles stephensi.
- Authors
Lombardo, F.; Nolan, T.; Lycett, G.; Lanfrancotti, A.; Stich, N.; Catteruccia, F.; Louis, C.; Coluzzi, M.; Arcà, B.
- Abstract
Regulatory regions driving gene expression in specific target organs of the African malaria vectorAnopheles gambiaeare of critical relevance for studies onPlasmodium–Anophelesinteractions as well as to devise strategies for blocking malaria parasite development in the mosquito. In order to identify an appropriate salivary gland promoter we analysed the transactivation properties of genomic fragments located just upstream of theAn. gambiaefemale salivary gland-specific genesAgApyandD7r4. An 800 bp fragment from theAgApygene directed specific expression of theLacZreporter gene in the salivary glands of transgenicAnopheles stephensi. However, expression levels were lower than expected and the transgene was expressed in the proximal-rather than in the distal-lateral lobes of female glands. Surprisingly, a promoter fragment from theD7r4gene conferred strong tissue-specific expression inDrosophila melanogasterbut only low transcription levels in transgenicAn. stephensi. These results imply a certain conservation of gland-specific control elements between the fruit fly and the mosquito suggesting that an increased degree of complexity, probably connected to the evolution of haematophagy, underlies the regulation of tissue-specific expression in mosquito female salivary glands.
- Subjects
GENE expression; SALIVARY glands; ANOPHELES; MALARIA; PLASMODIUM; PROTOZOAN diseases; DROSOPHILA
- Publication
Insect Molecular Biology, 2005, Vol 14, Issue 2, p207
- ISSN
0962-1075
- Publication type
Article
- DOI
10.1111/j.1365-2583.2004.00549.x