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- Title
Non-electrostatic interactions associated with aggregate formation between polyallylamine and Escherichia coli.
- Authors
Nakatsuji, Masatoshi; Sato, Natsuki; Sakamoto, Shiho; Watanabe, Koji; Teruuchi, Yoko; Takeuchi, Minoru; Inui, Takashi; Ishihara, Hideki
- Abstract
Bacterial aggregation by mixing with polymers is applied as pretreatment to identify pathogens in patients with infectious diseases. However, the detailed interaction between polymers and bacteria has yet to be fully understood. Here, we investigate the interaction between polyallylamine and Escherichia coli by isothermal titration calorimetry. Aggregation was observed at pH 10 and the binding was driven by favorable enthalpic gain such as the electrostatic interaction. Neither aggregation nor the apparent heat of binding was observed at pH 4.0, despite the strong positive charge of polyallylamine. These results suggest that intermolecular repulsive forces of the abundant positive charge of polyallylamine cause an increased loss of conformational entropy by binding. Non-electrostatic interaction plays a critical role for aggregation.
- Subjects
ESCHERICHIA coli; ISOTHERMAL titration calorimetry; INTERMOLECULAR forces; ELECTROSTATIC interaction; COMMUNICABLE diseases
- Publication
Scientific Reports, 2023, Vol 13, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-023-42120-2