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- Title
Drug-Resistant Polymorphisms and Copy Numbers in Plasmodium falciparum, Mozambique, 2015.
- Authors
Gupta, Himanshu; Macete, Eusebio; Bulo, Helder; Salvador, Crizolgo; Warsame, Marian; Carvalho, Eva; Ménard, Didier; Ringwald, Pascal; Bassat, Quique; Enosse, Sonia; Mayor, Alfredo
- Abstract
One of the fundamental steps toward malaria control is the use of antimalarial drugs. The success of antimalarial treatment can be affected by the presence of drug-resistant populations of Plasmodium falciparum. To assess resistance, we used molecular methods to examine 351 P. falciparum isolates collected from 4 sentinel sites in Mozambique for K13, pfmdr1, pfcrt, and pfdhps polymorphisms and for plasmepsin2 (pfpm2) and pfmdr1 copy numbers. We found multiple copies of pfpm2 in 1.1% of isolates. All isolates carried K13 wild-type alleles (3D7-like), except 4 novel polymorphisms (Leu619Leu, Phe656Ile, Val666Val, Gly690Gly). Prevalence of isolates with pfcrt mutant (K76T) allele was low (2.3%). Prevalence of isolates with pfdhps mutant alleles (A437G and K540E) was >80%, indicating persistence of sulfadoxine/pyrimethamine resistance; however, markers of artemisinin were absent, and markers of piperaquine resistance were low. Piperaquine resistance isolates may spread in Mozambique as dihydroartemisinin/piperaquine drug pressure increases.
- Subjects
MOZAMBIQUE; MALARIA treatment; ANTIMALARIALS; PLASMODIUM falciparum; DRUG resistance; ARTEMISININ; DRUG therapy for malaria; ALLELES; GENETIC polymorphisms; GENETICS; MALARIA; PROTOZOA; PHARMACODYNAMICS
- Publication
Emerging Infectious Diseases, 2018, Vol 24, Issue 1, p40
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2401.170864