We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Development of a Quantitative Determination Method for Polysorbate 80 in Biologicals by Size-Exclusion High-Performance Liquid Chromatography.
- Authors
Rounova, O. B.; Korotkov, M. G.; Ustinnikova, O. B.
- Abstract
Aquantitative determination method for polysorbate 80 in biological medicinal preparations using size-exclusion chromatography with UV detection was developed. OASIS HLB solid-phase extraction cartridges were used to extract polysorbate 80 from formulations with high protein concentrations. The HPLC separation was performed using a TSKgel G2000SWX column in isocratic mode. The mobile phase was phosphate buffer (0.1 M) containing sodium sulfate (0.1 M) and sodium dodecyl sulfate (0.1%). The developed method could be used for quantitative determination of polysorbate 80 in biological medicinal products.
- Subjects
HIGH performance liquid chromatography; POLYSORBATE 80; MICELLAR liquid chromatography; SODIUM dodecyl sulfate; SOLID phase extraction; SODIUM sulfate; QUANTITATIVE research
- Publication
Pharmaceutical Chemistry Journal, 2024, Vol 57, Issue 12, p2013
- ISSN
0091-150X
- Publication type
Article
- DOI
10.1007/s11094-024-03110-4