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- Title
Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish.
- Authors
Lagendijk, Anne Karine; Gomez, Guillermo A.; Sungmin Baek; Hesselson, Daniel; Hughes, William E.; Paterson, Scott; Conway, Daniel E.; Belting, Heinz-Georg; Affolter, Markus; Smith, Kelly A.; Schwartz, Martin A.; Yap, Alpha S.; Hogan, Benjamin M.
- Abstract
Forces play diverse roles in vascular development, homeostasis and disease. VE-cadherin at endothelial cell-cell junctions links the contractile acto-myosin cytoskeletons of adjacent cells, serving as a tension-transducer. To explore tensile changes across VE-cadherin in live zebrafish, we tailored an optical biosensor approach, originally established in vitro. We validate localization and function of a VE-cadherin tension sensor (TS) in vivo. Changes in tension across VE-cadherin observed using ratio-metric or lifetime FRET measurements reflect acto-myosin contractility within endothelial cells. Furthermore, we apply the TS to reveal biologically relevant changes in VE-cadherin tension that occur as the dorsal aorta matures and upon genetic and chemical perturbations during embryonic development.
- Subjects
CYTOSKELETON; CADHERINS; ZEBRA danio; ENDOTHELIAL cells; BIOSENSORS; VASCULAR endothelial growth factors
- Publication
Nature Communications, 2017, Vol 8, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-017-01325-6