We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh, India.
- Authors
Sharif, N. Mohammad; Sreedevi, B.; Chaitanya, R. K.; Sreenivasulu, D.
- Abstract
Aim: The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh. Materials and Methods: A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimicrobial resistance phenotypically by disc diffusion method and genotypically by polymerase chain reaction targeting blaTEM, blaSHV, blaOXA, blaCTX-M Group 1, 2, blaAmpC, blaACC and blaMOX genes. Results: A total of 33 Klebsiella and 29 Enterobacter isolates were recovered. Phenotypic beta-lactamase resistance was detected in 66.6% and 25% of Klebsiella and Enterobacter isolates, respectively, from healthy dogs and 66.6% and 60% of Klebsiella and Enterobacter isolates, respectively, from diarrheic dogs. Overall, incidence of extended-spectrum betalactamase (ESBL) phenotype was found to be 21.2% (7/33) in Klebsiella isolates, whereas none of the Enterobacter isolates exhibited ESBL phenotype. Predominant beta-lactamase genes detected in Klebsiella species include blaSHV (84.8%), followed by blaTEM (33.3%), blaCTX-M Group 1 (15.1%) and blaOXA (6.1%) gene. Predominant beta-lactamase genes detected in Enterobacter species include blaSHV (48.2%), followed by blaTEM (24.1%), blaAmpC (13.7%) and blaOXA (10.3%) gene. Conclusion: The present study highlighted alarming beta-lactamase resistance in Klebsiella and Enterobacter species of canine origin in India with due emphasis as indicators of antimicrobial resistance.
- Subjects
BETA-lactamase inhibitors; ANTI-infective agents; KLEBSIELLA infections; ENTEROBACTER; ENTEROBACTERIACEAE diseases; POLYMERASE chain reaction; VETERINARY therapeutics; DOG diseases
- Publication
Veterinary World, 2017, Vol 10, Issue 8, p950
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2017.950-954