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- Title
Molecular cloning and characterization of venom peptides from two Honeybee species (Apis cerana and Apis mellifera).
- Authors
Revataganv, Muttugoud; alakrishnan, N.; Srinivasan, M. R.; Mohankumar, S.
- Abstract
Two honey bee species (Apis cerana and Apis mellifera) are domesticated for the economic products like honey, royal jelly and venom. Three components of honey bee venom peptide viz., melittin, phospholipase A2 (PLA2), and secapin have proven effects in pharmaceutical and clinical applications and these components were cloned and characterized in the present study. Bee venom glands were dissected out for RNA isolation and cDNA was synthesized from the total RNA. PCR was carried out using gene-specific primers for melittin, PLA2, and secapin genes, using cDNA as template. PCR products were cloned in the TA-cloning vector and sequenced. Amino acid sequences were deduced from nucleic acid sequences and a phylogenetic tree was constructed to deduce the evolutionary relationship among the honey bee species. Crude bee venom was collected by electric milking method and dissolved in sterile water to separate the venom peptides by SDS-PAGE. Crude bee venom was tested against the rice pathogenic bacterium Xanthomonas oryzae pv. oryzae which causes bacterial blight disease of rice. Agar diffusion assay of bee venom was performed to confirm the antimicrobial properties of crude venom. Both A. cerana and A. mellifera venom showed antibacterial activity against X. oryzae pv. oryzae.
- Subjects
APIS cerana; MOLECULAR cloning; HONEYBEES; CONOTOXINS; BEE venom; VENOM; PEPTIDES
- Publication
Madras Agricultural Journal, 2019, Vol 106, Issue 7-9, p143
- ISSN
0024-9602
- Publication type
Article
- DOI
10.29321/MAJ 2019.000287